4awg

X-ray diffraction
2.6Å resolution

Influenza strain pH1N1 2009 polymerase subunit PA endonuclease in complex with diketo compound 3

Released:
Entry authors: Kowalinski E, Zubieta C, Wolkerstorfer A, Szolar OH, Ruigrok RW, Cusack S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 204 amino acids
Theoretical weight: 23.94 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-198; Coverage: 28%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 54.57Å b: 122.54Å c: 129.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.262
Expression system: Escherichia coli BL21(DE3)