4aw2

X-ray diffraction
1.7Å resolution

Crystal structure of CDC42 binding protein kinase alpha (MRCK alpha)

Released:
Source organism: Rattus norvegicus
Entry authors: Elkins JM, Muniz JRC, Tan I, Leung T, Lafanechere L, Prudent R, Abdul Azeez K, Szklarz M, Phillips C, Wang J, von Delft F, Bountra C, Edwards A, Knapp S

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase MRCK alpha Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 50.27 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O54874 (Residues: 2-424; Coverage: 24%)
Gene names: Cdc42bpa, Pk428
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 106.744Å b: 49.187Å c: 97.762Å
α: 90° β: 113.4° γ: 90°
R-values:
R R work R free
0.17 0.169 0.193
Expression system: Escherichia coli BL21(DE3)