X-ray diffraction
2.1Å resolution

Crystal structure of the tuberculosis drug target Decaprenyl- Phosphoryl-beta-D-Ribofuranose-2-oxidoreductase (DprE1) from Mycobacterium smegmatis


Function and Biology Details

Reaction catalysed:
Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Decaprenylphosphoryl-beta-D-ribose oxidase Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 51.19 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0R607 (Residues: 1-460; Coverage: 100%)
Gene names: LJ00_31545, MSMEG_6382, MSMEI_6214, dprE1
Sequence domains:

Ligands and Environments

Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 64.139Å b: 64.139Å c: 191.204Å
α: 90° β: 90° γ: 120°
R R work R free
0.206 0.202 0.271
Expression system: Escherichia coli BL21(DE3)