› 4au5

X-ray diffraction
3.7Å resolution

Structure of the NhaA dimer, crystallised at low pH

Released:
DOI: 10.2210/pdb4au5/pdb
PDB 4au5 coloured by chain and viewed from the front.
PDB 4au5 coloured by chain and viewed from the side.
PDB 4au5 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.
Lee C, Yashiro S, Dotson DL, Uzdavinys P, Iwata S, Sansom MS, von Ballmoos C, Beckstein O, Drew D, Cameron AD
J. Gen. Physiol. 144 529-44 (2014)
PMID: 25422503

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) antiporter NhaA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 401 amino acids
Theoretical weight: 42.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13738 (Residues: 1-388; Coverage: 100%)
Gene names: JW0018, ant, b0019, nhaA
Sequence domains: Na+/H+ antiporter 1
Structure domains: Na+/H+ antiporter like domain

Ligands and Environments

2 bound ligands:
Ligand LMU 1 x LMU
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 115.8Å b: 100.56Å c: 141.612Å
α: 90° β: 97° γ: 90°
R-values:
R R work R free
0.319 0.318 0.342
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

General principles of secondary active transporter function.
Beckstein et al. (2022)
6 more

10 mentions without citation

The role of interfacial lipids in stabilizing membrane protein oligomers.
Gupta et al. (2017)
9 more