4ato

X-ray diffraction
2.2Å resolution

New insights into the mechanism of bacterial Type III toxin-antitoxin systems: selective toxin inhibition by a non-coding RNA pseudoknot

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endoribonuclease ToxN Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 22.93 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3YN09 (Residues: 1-194; Coverage: 100%)
Gene names: pAW63_066, toxN
Sequence domains: Toxin ToxN, type III toxin-antitoxin system
Structure domains: Thiol Ester Dehydrase; Chain A
TOXI Chain: G
Molecule details ›
Chain: G
Length: 34 nucleotides
Theoretical weight: 10.99 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
Sequence domains: ToxI antitoxin

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P6
Unit cell:
a: 127.1Å b: 127.1Å c: 37.735Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.192
Expression system: Escherichia coli