4atn

X-ray diffraction
1.95Å resolution

Crystal structure of C2498 2'-O-ribose methyltransferase RlmM from Escherichia coli

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + cytidine(2498) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(2498) in 23S rRNA 
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase M Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 43.09 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADR6 (Residues: 1-366; Coverage: 100%)
Gene names: JW2777, b1976, b2806, rlmM, ygdE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P3121
Unit cell:
a: 92.09Å b: 92.09Å c: 83.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.206
Expression system: Escherichia coli BL21(DE3)