4at9

X-ray diffraction
2.8Å resolution

Crystal structure of the NF90-NF45 dimerisation domain complex with UTP

Released:
Source organism: Mus musculus
Entry authors: Wolkowicz UM, Cook AG

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interleukin enhancer-binding factor 2 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 40.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9CXY6 (Residues: 29-390; Coverage: 93%)
Gene names: Ilf2, Nf45
Sequence domains: DZF domain
Structure domains:
Interleukin enhancer-binding factor 3 Chain: B
Molecule details ›
Chain: B
Length: 383 amino acids
Theoretical weight: 42.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Z1X4 (Residues: 1-381; Coverage: 42%)
Gene name: Ilf3
Sequence domains: DZF domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I222
Unit cell:
a: 83.849Å b: 133.516Å c: 177.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.236 0.263
Expression system: Escherichia coli BL21