› 4as5

X-ray diffraction
2.43Å resolution

Structure of mouse inositol monophosphatase 1

Released:
DOI: 10.2210/pdb4as5/pdb
PDB entry 4as5 coloured by chain, front view.
PDB entry 4as5 coloured by chain, side view.
PDB entry 4as5 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens.
Singh N, Halliday AC, Knight M, Lack NA, Lowe E, Churchill GC
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1149-52 (2012)
PMID: 23027737

Function and Biology Details

Reactions catalysed: 
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Alpha-D-galactose 1-phosphate + H(2)O = D-galactose + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol monophosphatase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 277 amino acids
Theoretical weight: 30.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O55023 (Residues: 1-277; Coverage: 100%)
Gene name: Impa1
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PO4 4 x PO4
Ligand MG 12 x MG
Ligand IOD 9 x IOD
Ligand EDO 5 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 86.785Å b: 81.49Å c: 97.139Å
α: 90° β: 113.77° γ: 90°
R-values:
R R work R free
0.163 0.16 0.214
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Structural elucidation of the binding site and mode of inhibition of Li(+) and Mg(2+) in inositol monophosphatase.
Dutta et al. (2014)
5 more

2 mentions without citation

Defective craniofacial development and brain function in a mouse model for depletion of intracellular inositol synthesis.
Ohnishi et al. (2014)
1 more