4aq7

X-ray diffraction
2.5Å resolution

Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucyl-adenylate analogue in the aminoacylation conformation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Leucine--tRNA ligase Chains: A, D
Molecule details ›
Chains: A, D
Length: 880 amino acids
Theoretical weight: 99.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07813 (Residues: 1-860; Coverage: 100%)
Gene names: JW0637, b0642, leuS
Sequence domains:
Structure domains:
E. COLI TRNALEU UAA ISOACCEPTOR Chains: B, E
Molecule details ›
Chains: B, E
Length: 87 nucleotides
Theoretical weight: 28.05 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 158.66Å b: 69.2Å c: 228.84Å
α: 90° β: 104.35° γ: 90°
R-values:
R R work R free
0.191 0.188 0.25
Expression systems:
  • Escherichia coli BL21
  • Not provided