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X-ray diffraction
2.3Å resolution

B. subtilis dUTPase YncF in complex with dU PPi and Mg in H32

Released:
Source organism: Bacillus subtilis
Primary publication:
Tying down the arm in Bacillus dUTPase: structure and mechanism.
Acta Crystallogr. D Biol. Crystallogr. 69 1367-80 (2013)
PMID: 23897460

Function and Biology Details

Reaction catalysed:
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase YncF Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 144 amino acids
Theoretical weight: 16.43 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31801 (Residues: 1-144; Coverage: 100%)
Gene names: BSU17660, yncF
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: R32
Unit cell:
a: 146.628Å b: 146.628Å c: 144.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.163 0.219
Expression system: Escherichia coli