X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE Covalent Intermediate Complex with 5-fluoro-glucosyl- fluoride


Function and Biology Details

Reaction catalysed:
Linear alpha-glucan = (n-1) 1,5-anhydro-D-fructose + D-glucose
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-1,4-glucan lyase isozyme 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1027 amino acids
Theoretical weight: 116.06 KDa
Source organism: Gracilariopsis lemaneiformis
Expression system: Ogataea polymorpha RB11
  • Canonical: Q9STC1 (Residues: 62-1088; Coverage: 94%)
Gene name: Ag111
Sequence domains: Glycosyl hydrolases family 31
Structure domains:

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 91.643Å b: 97.008Å c: 135.708Å
α: 80.38° β: 83.11° γ: 85.22°
R R work R free
0.223 0.22 0.267
Expression system: Ogataea polymorpha RB11