4alp

X-ray diffraction
1.48Å resolution

The Lin28b Cold shock domain in complex with hexauridine

Released:
Primary publication:
The Lin28 cold-shock domain remodels pre-let-7 microRNA.
Nucleic Acids Res 40 7492-506 (2012)
PMID: 22570413

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero decamer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-110088 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein lin-28 homolog B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 90 amino acids
Theoretical weight: 9.94 KDa
Source organism: Xenopus tropicalis
Expression system: Escherichia coli
UniProt:
  • Canonical: B4F6I0 (Residues: 27-114; Coverage: 35%)
Gene names: LIN-28, csdd2, lin28b
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins
HEXA URIDINE Chain: E
Molecule details ›
Chain: E
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY
Spacegroup: P21212
Unit cell:
a: 95.982Å b: 61.166Å c: 63.219Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.188
Expression systems:
  • Escherichia coli
  • Not provided