X-ray diffraction
2Å resolution

Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant

Source organism: Bacillus subtilis
Entry authors: Silva CS, Chen Z, Durao P, Pereira MM, Todorovic S, Hildebrandt P, Martins LO, Lindley PF, Bento I

Function and Biology Details

Reactions catalysed:
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Laccase Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 58.56 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
  • Canonical: P07788 (Residues: 1-513; Coverage: 100%)
Gene names: BSU06300, cotA, pig
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 101.898Å b: 101.898Å c: 136.931Å
α: 90° β: 90° γ: 120°
R R work R free
0.179 0.177 0.215
Expression system: Escherichia coli