4aj5

X-ray diffraction
3.32Å resolution

Crystal structure of the Ska core complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Spindle and kinetochore-associated protein 3 Chains: 1, 2, 3, 4, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, 3, 4, U, V, W, X, Y, Z
Length: 101 amino acids
Theoretical weight: 11.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IX90 (Residues: 1-101; Coverage: 25%)
  • Best match: Q8IX90-2 (Residues: 1-34)
Gene names: C13orf3, RAMA1, SKA3
Spindle and kinetochore-associated protein 1 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 91 amino acids
Theoretical weight: 10.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96BD8 (Residues: 1-91; Coverage: 36%)
Gene names: C18orf24, SKA1
Spindle and kinetochore-associated protein 2 Chains: K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: K, L, M, N, O, P, Q, R, S, T
Length: 123 amino acids
Theoretical weight: 14.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WVK7 (Residues: 1-121; Coverage: 100%)
Gene names: FAM33A, SKA2
Sequence domains: Spindle and kinetochore-associated protein 2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 174.4Å b: 193.121Å c: 198.267Å
α: 90° β: 95.21° γ: 90°
R-values:
R R work R free
0.237 0.234 0.28
Expression system: Escherichia coli BL21