› 4aih

X-ray diffraction
2.4Å resolution

Crystal structure of RovA from Yersinia in its free form

Released:
DOI: 10.2210/pdb4aih/pdb
PDB entry 4aih coloured by chain, front view.
PDB entry 4aih coloured by chain, side view.
PDB entry 4aih coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis YPIII
Primary publication:
Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
Quade N, Mendonca C, Herbst K, Heroven AK, Ritter C, Heinz DW, Dersch P
J Biol Chem 287 35796-803 (2012)
PMID: 22936808

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator SlyA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 151 amino acids
Theoretical weight: 17.23 KDa
Source organism: Yersinia pseudotuberculosis YPIII
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B1JJ73 (Residues: 1-143; Coverage: 100%)
Gene names: YPK_1876, slyA
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 67.16Å b: 74.78Å c: 181.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.266
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Bacterial Stress Responses during Host Infection.
Fang et al. (2016)
8 more

2 mentions without citation

Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
Quade et al. (2012)
1 more