› 4ag5

X-ray diffraction
2.45Å resolution

Structure of VirB4 of Thermoanaerobacter pseudethanolicus

Released:
DOI: 10.2210/pdb4ag5/pdb
PDB entry 4ag5 coloured by chain, front view.
PDB entry 4ag5 coloured by chain, side view.
PDB entry 4ag5 coloured by chain, top view.
Source organism: Thermoanaerobacter pseudethanolicus
Primary publication:
Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system.
Walldén K, Williams R, Yan J, Lian PW, Wang L, Thalassinos K, Orlova EV, Waksman G
Proc Natl Acad Sci U S A 109 11348-53 (2012)
PMID: 22745169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TraG P-loop domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 44.17 KDa
Source organism: Thermoanaerobacter pseudethanolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0KAW2 (Residues: 203-594; Coverage: 66%)
Gene name: Teth39_0060
Sequence domains: TraG P-loop domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand GOL 3 x GOL
Ligand ADP 2 x ADP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 108.08Å b: 110.8Å c: 156.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
14 more

12 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
11 more