› 4afk

X-ray diffraction
1.9Å resolution

In meso structure of alginate transporter, AlgE, from Pseudomonas aeruginosa, PAO1

Released:
DOI: 10.2210/pdb4afk/pdb
PDB entry 4afk coloured by chain, front view.
PDB entry 4afk coloured by chain, side view.
PDB entry 4afk coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa.
Tan J, Rouse SL, Li D, Pye VE, Vogeley L, Brinth AR, El Arnaout T, Whitney JC, Howell PL, Sansom MS, Caffrey M
Acta Crystallogr D Biol Crystallogr 70 2054-68 (2014)
PMID: 25084326

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate production protein AlgE Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.2 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18895 (Residues: 33-490; Coverage: 100%)
Gene names: PA3544, alg76, algE
Sequence domains: Alginate export
Structure domains: Porin

Ligands and Environments

7 bound ligands:
Ligand CA 1 x CA
Ligand FLC 1 x FLC
Ligand LDA 15 x LDA
Ligand NA 2 x NA
Ligand 78M 3 x 78M
Ligand 78N 3 x 78N
Ligand PE5 2 x PE5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 57.253Å b: 74.416Å c: 115.529Å
α: 90° β: 101.56° γ: 90°
R-values:
R R work R free
0.166 0.163 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

A molecular description of cellulose biosynthesis.
McNamara et al. (2015)
2 more

9 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
8 more