Structure analysis

Selenomethionine derivative of the GH99 endo-alpha-mannosidase from Bacteroides thetaiotaomicron

X-ray diffraction
1.69Å resolution
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1
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Multimeric state: monomeric
Accessible surface area: 13700 Å2
Buried surface area: 750 Å2
Dissociation area: 50 Å2
Dissociation energy (ΔGdiss): -1 kcal/mol
Dissociation entropy (TΔSdiss): 1 kcal/mol
Interface energy (ΔGint): -1 kcal/mol
Symmetry number: 1
Assembly 2 (preferred)
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Multimeric state: monomeric
Accessible surface area: 13400 Å2
Buried surface area: 1100 Å2
Dissociation area: 150 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 1 kcal/mol
Interface energy (ΔGint): -4 kcal/mol
Symmetry number: 1

Macromolecules

Chains: A, B
Length: 382 amino acids
Theoretical weight: 43.64 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A109 (Residues: 1-382; Coverage: 100%)
Gene name: BT_3862
Pfam: Glycosyl hydrolase family 99
InterPro: Glycosyl hydrolase family 99
CATH: Glycosidases

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