› 4acp

X-ray diffraction
2.49Å resolution

Deactivation of human IgG1 Fc by endoglycosidase treatment

Released:
DOI: 10.2210/pdb4acp/pdb
PDB entry 4acp coloured by chain, front view.
PDB entry 4acp coloured by chain, side view.
PDB entry 4acp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selective deactivation of serum IgG: a general strategy for the enhancement of monoclonal antibody receptor interactions.
Baruah K, Bowden TA, Krishna BA, Dwek RA, Crispin M, Scanlan CN
J Mol Biol 420 1-7 (2012)
PMID: 22484364

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 101-329; Coverage: 57%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 69.29Å b: 110.857Å c: 77.508Å
α: 90° β: 107.92° γ: 90°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Homo sapiens

Quick links

Citations

13 review citations

Emerging principles for the therapeutic exploitation of glycosylation.
Dalziel et al. (2014)
12 more

5 mentions without citation

The Structural Role of Antibody N-Glycosylation in Receptor Interactions.
Subedi et al. (2015)
4 more