4ab1

X-ray diffraction
2.2Å resolution

Recombinant Human Carboxylesterase 1 from whole Cabbage Loopers

Released:
Source organism: Homo sapiens
Primary publication:
Structure of recombinant human carboxylesterase 1 isolated from whole cabbage looper larvae.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68 269-72 (2012)
PMID: 22442219

Function and Biology Details

Reactions catalysed:
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
A steryl ester + H(2)O = a sterol + a fatty acid
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Liver carboxylesterase 1 Chain: A
Molecule details ›
Chain: A
Length: 532 amino acids
Theoretical weight: 58.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P23141 (Residues: 21-553; Coverage: 97%)
Gene names: CES1, CES2, SES1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6322
Unit cell:
a: 114.843Å b: 114.843Å c: 177.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.236
Expression system: Trichoplusia ni