› 4aan

X-ray diffraction
1.22Å resolution

MacA wild-type fully reduced

Released:
DOI: 10.2210/pdb4aan/pdb
PDB entry 4aan coloured by chain, front view.
PDB entry 4aan coloured by chain, side view.
PDB entry 4aan coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Primary publication:
MacA is a second cytochrome c peroxidase of Geobacter sulfurreducens.
Seidel J, Hoffmann M, Ellis KE, Seidel A, Spatzal T, Gerhardt S, Elliott SJ, Einsle O
Biochemistry 51 2747-56 (2012)
PMID: 22417533

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 36.81 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q74FY6 (Residues: 23-346; Coverage: 94%)
Gene names: GSU0466, macA
Sequence domains:
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEC 2 x HEC
2 bound ligands:
Ligand CA 1 x CA
Ligand BU3 4 x BU3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 95.6Å b: 46.97Å c: 77.93Å
α: 90° β: 90.94° γ: 90°
R-values:
R R work R free
0.148 0.147 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Multi-heme proteins: nature's electronic multi-purpose tool.
Bewley et al. (2013)
2 more

1 mention without citation

MacA is a second cytochrome c peroxidase of Geobacter sulfurreducens.
Seidel et al. (2012)