4aae

X-ray diffraction
2.6Å resolution

Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3')

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 17.6 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-154; Coverage: 94%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
24MER DNA Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.39 KDa
Source organism: synthetic construct
Expression system: Not provided
24MER DNA Chain: G
Molecule details ›
Chain: G
Length: 24 nucleotides
Theoretical weight: 7.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS
Unit cell:
a: 49.15Å b: 70.34Å c: 172.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.175 0.242
Expression systems:
  • Escherichia coli
  • Not provided