4a4i

X-ray diffraction
1.95Å resolution

Crystal structure of the human Lin28b cold shock domain

Released:
Source organism: Homo sapiens
Primary publication:
The Lin28 cold-shock domain remodels pre-let-7 microRNA.
Nucleic Acids Res. (2012)
PMID: 22570413

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein lin-28 homolog B Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6ZN17 (Residues: 24-111; Coverage: 35%)
Gene names: CSDD2, LIN28B
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 46.181Å b: 62.477Å c: 77.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.235
Expression system: Escherichia coli BL21(DE3)