› 4a4i

X-ray diffraction
1.95Å resolution

Crystal structure of the human Lin28b cold shock domain

Released:
DOI: 10.2210/pdb4a4i/pdb
PDB 4a4i coloured by chain and viewed from the front.
PDB 4a4i coloured by chain and viewed from the side.
PDB 4a4i coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
The Lin28 cold-shock domain remodels pre-let-7 microRNA.
Mayr F, Schütz A, Döge N, Heinemann U
Nucleic Acids Res. (2012)
PMID: 22570413

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein lin-28 homolog B Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6ZN17 (Residues: 24-111; Coverage: 35%)
Gene names: CSDD2, LIN28B
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 46.181Å b: 62.477Å c: 77.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

LIN28 Family in Testis: Control of Cell Renewal, Maturation, Fertility and Aging.
Krsnik et al. (2022)
14 more

6 mentions without citation

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
5 more