Structure analysis

RNA Polymerase II initial transcribing complex with a 4nt DNA-RNA hybrid and soaked with AMPCPP

X-ray diffraction
3.9Å resolution
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero pentadecamer (preferred)
Entry contents: 12 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentadecamer
Accessible surface area: 151155.33 Å2
Buried surface area: 72218.22 Å2
Dissociation area: 1,809.43 Å2
Dissociation energy (ΔGdiss): 8.05 kcal/mol
Dissociation entropy (TΔSdiss): 15.12 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-117197

Macromolecules

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Chain: D
Length: 221 amino acids
Theoretical weight: 25.45 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P20433 (Residues: 1-221; Coverage: 100%)
Gene names: J0654, RPB4, YJL140W
Pfam: RNA polymerase Rpb4
InterPro:
CATH: Growth Hormone; Chain: A;

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Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P20436 (Residues: 1-146; Coverage: 100%)
Gene names: RPB8, YOR224C, YOR50-14
Pfam: RNA polymerase Rpb8
InterPro:
CATH: Nucleic acid-binding proteins

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Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P22139 (Residues: 1-70; Coverage: 100%)
Gene names: RPB10, YOR210W
Pfam: RNA polymerases N / 8 kDa subunit
InterPro:
CATH: Homeodomain-like

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Name: 5'-D(*AP*AP*GP*TP*AP*CP*TP)-3'
Representative chains: N
Length: 14 nucleotides
Theoretical weight: 4.29 KDa

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Name: 5'-D(*AP*GP*CP*TP*CP*AP*AP*GP*TP*AP*CP*TP*TP*TP* TP*TP*CP*CP*BRUP*GP*GP*TP*CP*AP*TP*T)-3'
Representative chains: T
Length: 26 nucleotides
Theoretical weight: 7.98 KDa

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Name: 5'-R(*AP*GP*GP*A)-3'
Representative chains: P
Length: 4 nucleotides
Theoretical weight: 1.3 KDa

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