PDBe 4a12

X-ray diffraction
3.15Å resolution

Structure of the global transcription regulator FapR from Staphylococcus aureus in complex with DNA operator

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor FapR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 22.12 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2FZ56 (Residues: 1-190; Coverage: 100%)
Gene names: SAOUHSC_01196, fapR
Sequence domains: Thioesterase superfamily
Structure domains:
FAPR PROMOTER Chain: F
Molecule details ›
Chain: F
Length: 40 nucleotides
Theoretical weight: 12.2 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
FAPR PROMOTER Chain: G
Molecule details ›
Chain: G
Length: 40 nucleotides
Theoretical weight: 12.42 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 114.69Å b: 249.94Å c: 179.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.201
Expression systems:
  • Escherichia coli BL21
  • Not provided