› 4a0t

X-ray diffraction
1.9Å resolution

Structure of the carboxy-terminal domain of bacteriophage T7 fibre gp17 containing residues 371-553.

Released:
DOI: 10.2210/pdb4a0t/pdb
PDB entry 4a0t coloured by chain, front view.
PDB entry 4a0t coloured by chain, side view.
PDB entry 4a0t coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Structure of the receptor-binding carboxy-terminal domain of bacteriophage T7 tail fibers.
Garcia-Doval C, van Raaij MJ
Proc Natl Acad Sci U S A 109 9390-5 (2012)
PMID: 22645347

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tail fiber protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 228 amino acids
Theoretical weight: 25.35 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03748 (Residues: 370-553, 372-553; Coverage: 33%)
Gene name: 17
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TMO 3 x TMO
Ligand TRS 1 x TRS
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 61.24Å b: 86.04Å c: 118.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Molecular architecture of tailed double-stranded DNA phages.
Fokine et al. (2014)
10 more

8 mentions without citation

Engineering Phage Host-Range and Suppressing Bacterial Resistance through Phage Tail Fiber Mutagenesis.
Yehl et al. (2019)
7 more