› 4zyr

X-ray diffraction
3.31Å resolution

Crystal structure of E. coli Lactose permease G46W/G262W bound to p-nitrophenyl alpha-D-galactopyranoside (alpha-NPG)

Released:
DOI: 10.2210/pdb4zyr/pdb
PDB entry 4zyr coloured by chain, front view.
PDB entry 4zyr coloured by chain, side view.
PDB entry 4zyr coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site.
Kumar H, Finer-Moore JS, Kaback HR, Stroud RM
Proc Natl Acad Sci U S A 112 9004-9 (2015)
PMID: 26157133

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 46.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02920 (Residues: 1-417; Coverage: 100%)
Gene names: JW0334, b0343, lacY
Sequence domains: LacY proton/sugar symporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

2 bound ligands:
Ligand 9PG 2 x 9PG
Ligand BNG 2 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 103.15Å b: 121.99Å c: 266.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.259 0.278
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
4 more

10 mentions without citation

It takes two to tango: The dance of the permease.
Kaback et al. (2019)
9 more