4zul

X-ray diffraction
1.76Å resolution

Structure ALDH7A1 complexed with alpha-aminoadipate

Released:
Source organism: Homo sapiens
Primary publication:
Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
OpenAccess logo Biochemistry (2015)
PMID: 26260980

Function and Biology Details

Reactions catalysed:
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
(1a) (S)-2-amino-6-oxohexanoate = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H(2)O (spontaneous)
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-aminoadipic semialdehyde dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 513 amino acids
Theoretical weight: 55.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49419 (Residues: 29-539; Coverage: 95%)
  • Best match: P49419-2 (Residues: 1-511)
Gene names: ALDH7A1, ATQ1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 154.43Å b: 162.534Å c: 158.678Å
α: 90° β: 95.04° γ: 90°
R-values:
R R work R free
0.17 0.168 0.206
Expression system: Escherichia coli BL21(DE3)