› 4zj1

X-ray diffraction
1.54Å resolution

Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli : V216AcrF mutant

Released:
DOI: 10.2210/pdb4zj1/pdb
PDB entry 4zj1 coloured by chain, front view.
PDB entry 4zj1 coloured by chain, side view.
PDB entry 4zj1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Exploring the potential impact of an expanded genetic code on protein function.
Xiao H, Nasertorabi F, Choi SH, Han GW, Reed SA, Stevens RC, Schultz PG
Proc Natl Acad Sci U S A 112 6961-6 (2015)
PMID: 26038548

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase TEM Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P62593 (Residues: 1-286; Coverage: 100%)
Gene names: bla, blaT-3, blaT-4, blaT-5, blaT-6
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand MES 1 x MES
Ligand PEG 1 x PEG
1 modified residue:
Ligand 4OU 1 x 4OU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 45.47Å b: 47.24Å c: 128.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.17
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Expanding and reprogramming the genetic code.
Chin JW. (2017)
10 more

5 mentions without citation

At the Interface of Chemical and Biological Synthesis: An Expanded Genetic Code.
Xiao et al. (2016)
4 more