PDBe 4zb4

X-ray diffraction
2.3Å resolution

Spliceosome component

Released:
Entry authors: Rocha de Moura T, Pena P

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-processing factor 19 Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.69 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32523 (Residues: 144-503; Coverage: 72%)
Gene names: PRP19, PSO4, YLL036C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 56.22Å b: 100.14Å c: 65.34Å
α: 90° β: 92.33° γ: 90°
R-values:
R R work R free
0.198 0.196 0.24
Expression system: Escherichia coli BL21(DE3)