4z3d

X-ray diffraction
1.8Å resolution

Human carbonyl reductase 1 with glutathione in a protective configuration

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
R-CHOH-R' + NADP(+) = R-CO-R' + NADPH
(5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP(+) = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH
(13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprost-13-enoate + NADP(+) = (13E)-11-alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonyl reductase [NADPH] 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 30.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16152 (Residues: 2-277; Coverage: 100%)
Gene names: CBR, CBR1, CRN, SDR21C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 55.567Å b: 57.18Å c: 99.453Å
α: 82.99° β: 87.32° γ: 85.66°
R-values:
R R work R free
0.183 0.182 0.212
Expression system: Escherichia coli