PDBe 4z2b

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2',5'-phosphodiesterase 12 Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 51.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6L8Q7 (Residues: 155-609; Coverage: 75%)
Gene name: PDE12
Sequence domains: Endonuclease/Exonuclease/phosphatase family

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 56.309Å b: 66.532Å c: 124.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.218
Expression system: Escherichia coli BL21(DE3)