› 4yr1

X-ray diffraction
2.24Å resolution

Crystal Structure of E. Coli Alkaline Phosphatase D101A/D153A in complex with inorganic phosphate

Released:
DOI: 10.2210/pdb4yr1/pdb
PDB entry 4yr1 coloured by chain, front view.
PDB entry 4yr1 coloured by chain, side view.
PDB entry 4yr1 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site.
Sunden F, Peck A, Salzman J, Ressl S, Herschlag D
Elife 4 (2015)
PMID: 25902402

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 443 amino acids
Theoretical weight: 46.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 31-471; Coverage: 98%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand PO4 2 x PO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6322
Unit cell:
a: 161.42Å b: 161.42Å c: 140.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.217 0.259
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Promiscuity in the Enzymatic Catalysis of Phosphate and Sulfate Transfer.
Pabis et al. (2016)
2 more

1 mention without citation

Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site.
Sunden et al. (2015)