4yoc

X-ray diffraction
2.92Å resolution

Crystal Structure of human DNMT1 and USP7/HAUSP complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA (cytosine-5)-methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 1004 amino acids
Theoretical weight: 113.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26358 (Residues: 600-1600; Coverage: 62%)
Gene names: AIM, CXXC9, DNMT, DNMT1
Sequence domains:
Ubiquitin carboxyl-terminal hydrolase 7 Chain: C
Molecule details ›
Chain: C
Length: 548 amino acids
Theoretical weight: 64.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93009 (Residues: 560-1102; Coverage: 49%)
Gene names: HAUSP, USP7
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 110.231Å b: 111.619Å c: 163.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.258
Expression system: Escherichia coli