4ykl

X-ray diffraction
2.25Å resolution

Function and Biology Details

Reactions catalysed:
(DNA)-3'-diphospho-5'-guanosine + H(2)O = (DNA)-3'-phosphate + GMP
Adenosine-5'-diphospho-5'-(DNA) + H(2)O = AMP + phospho-5'-(DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Aprataxin-like protein Chain: B
Molecule details ›
Chain: B
Length: 200 amino acids
Theoretical weight: 23.23 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O74859 (Residues: 33-232; Coverage: 86%)
Gene names: SPCC18.09c, hnt3
Sequence domains:
Structure domains: HIT-like
DNA (5'-D(*GP*AP*AP*TP*CP*AP*TP*AP*AP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P321
Unit cell:
a: 119.083Å b: 119.083Å c: 36.564Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.18 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided