4yg2

X-ray diffraction
3.7Å resolution

X-ray crystal structur of Escherichia coli RNA polymerase sigma70 holoenzyme

Released:
Primary publication:
X-ray crystal structure of Escherichia coli RNA polymerase σ70 holoenzyme.
J. Biol. Chem. 288 9126-34 (2013)
PMID: 23389035

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7Z6 (Residues: 1-329; Coverage: 100%)
Gene names: ECs4160, Z4665, rpoA
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chains: C, I
Molecule details ›
Chains: C, I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V4 (Residues: 1-1342; Coverage: 100%)
Gene names: ECs4910, Z5560, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chains: D, J
Molecule details ›
Chains: D, J
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8T8 (Residues: 1-1407; Coverage: 100%)
Gene names: ECs4911, Z5561, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chains: E, K
Molecule details ›
Chains: E, K
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A802 (Residues: 1-91; Coverage: 100%)
Gene names: ECs4524, Z5075, rpoZ
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
RNA polymerase sigma factor RpoD Chains: F, L
Molecule details ›
Chains: F, L
Length: 613 amino acids
Theoretical weight: 70.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00579 (Residues: 1-613; Coverage: 100%)
Gene names: JW3039, alt, b3067, rpoD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 187.308Å b: 205.901Å c: 309.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.26
Expression system: Escherichia coli BL21(DE3)