4yex

X-ray diffraction
3.2Å resolution

HUaa-19bp

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein HU-alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 90 amino acids
Theoretical weight: 9.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACF0 (Residues: 1-90; Coverage: 100%)
Gene names: JW3964, b4000, hupA
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
synthetic DNA strand Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.31 KDa
Source organism: Escherichia coli
Expression system: Not provided
synthetic DNA strand Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: C2
Unit cell:
a: 105.958Å b: 49.951Å c: 62.633Å
α: 90° β: 114.7° γ: 90°
R-values:
R R work R free
0.22 0.218 0.263
Expression systems:
  • Escherichia coli
  • Not provided