PDBe 4y8w

X-ray diffraction
2.64Å resolution

Crystal Structure of Human Cytochrome P450 21A2 Progesterone Complex


Function and Biology Details

Reaction catalysed:
A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Steroid 21-hydroxylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 482 amino acids
Theoretical weight: 54.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P08686 (Residues: 29-494; Coverage: 94%)
Gene names: CYP21, CYP21A2, CYP21B
Structure domains: Cytochrome P450

Ligands and Environments

Cofactor: Ligand HEM 3 x HEM
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 150.383Å b: 86.86Å c: 108.923Å
α: 90° β: 102.11° γ: 90°
R R work R free
0.235 0.231 0.281
Expression system: Escherichia coli BL21(DE3)