4y5o

X-ray diffraction
2.35Å resolution

CCM2 HHD in complex with MEKK3 NPB1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cerebral cavernous malformations 2 protein Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BSQ5 (Residues: 283-379; Coverage: 22%)
Gene names: C7orf22, CCM2, PP10187
Structure domains: Paired amphipathic helix 2 (pah2 repeat)
Mitogen-activated protein kinase kinase kinase 3 Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 14.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99759 (Residues: 1-124; Coverage: 20%)
Gene names: MAP3K3, MAPKKK3, MEKK3
Sequence domains: PB1 domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 43.31Å b: 45.349Å c: 61.533Å
α: 90° β: 94.95° γ: 90°
R-values:
R R work R free
0.209 0.208 0.232
Expression system: Escherichia coli BL21(DE3)