PDBe 4xsx

X-ray diffraction
3.71Å resolution

Crystal structure of CBR 703 bound to Escherichia coli RNA polymerase holoenzyme

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 239 amino acids
Theoretical weight: 26.46 KDa
Source organism: Escherichia coli O139:H28 str. E24377A
UniProt:
  • Canonical: A7ZSI4 (Residues: 1-234; Coverage: 71%)
Gene names: EcE24377A_3778, rpoA
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chains: C, I
Molecule details ›
Chains: C, I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli O139:H28 str. E24377A
UniProt:
  • Canonical: A7ZUK1 (Residues: 1-1342; Coverage: 100%)
Gene names: EcE24377A_4528, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chains: D, J
Molecule details ›
Chains: D, J
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli O139:H28 str. E24377A
UniProt:
  • Canonical: A7ZUK2 (Residues: 1-1407; Coverage: 100%)
Gene names: EcE24377A_4529, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chains: E, K
Molecule details ›
Chains: E, K
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Citrobacter koseri ATCC BAA-895
UniProt:
  • Canonical: A8ARN6 (Residues: 1-91; Coverage: 100%)
Gene names: CKO_05106, rpoZ
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
RNA polymerase sigma factor RpoD Chains: F, L
Molecule details ›
Chains: F, L
Length: 522 amino acids
Theoretical weight: 60.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00579 (Residues: 92-613; Coverage: 85%)
Gene names: JW3039, alt, b3067, rpoD
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 187.025Å b: 206.914Å c: 310.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.273
Expression system: Escherichia coli BL21(DE3)