4xr0

X-ray diffraction
2.8Å resolution

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- G/T mismatch.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA replication terminus site-binding protein Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16525 (Residues: 1-309; Coverage: 100%)
Gene names: JW1602, b1610, tau, tus
Sequence domains: DNA replication terminus site-binding protein (Ter protein)
Structure domains:
DNA (5'-D(*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*AP*TP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P41212
Unit cell:
a: 64.523Å b: 64.523Å c: 248.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.296
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided