4xqk

X-ray diffraction
2.7Å resolution

ATP-dependent Type ISP restriction-modification enzyme LlaBIII bound to DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
LlaBIII Chains: A, B
Molecule details ›
Chains: A, B
Length: 1578 amino acids
Theoretical weight: 180.18 KDa
Source organism: Lactococcus lactis subsp. cremoris
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KL05 (Residues: 1-1578; Coverage: 100%)
Sequence domains:
DNA (28-MER) Chains: C, E
Molecule details ›
Chains: C, E
Length: 28 nucleotides
Theoretical weight: 8.54 KDa
Source organism: unidentified
Expression system: Not provided
DNA (28-MER) Chains: D, F
Molecule details ›
Chains: D, F
Length: 28 nucleotides
Theoretical weight: 8.67 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 62.88Å b: 112.66Å c: 146.21Å
α: 103.3° β: 90.88° γ: 105.79°
R-values:
R R work R free
0.221 0.218 0.26
Expression systems:
  • Escherichia coli
  • Not provided