4xo1

X-ray diffraction
1.8Å resolution

crystal structure of Se-Met GnsA with double mutations

Released:
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of GnsA from Escherichia coli.
Biochem. Biophys. Res. Commun. 462 1-7 (2015)
PMID: 25839658

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein GnsA Chain: A
Molecule details ›
Chain: A
Length: 60 amino acids
Theoretical weight: 7.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC92 (Residues: 1-57; Coverage: 100%)
Gene names: JW0976, b4517, gnsA, yccL
Sequence domains: GnsA/GnsB toxin of bacterial toxin-antitoxin system

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: C2221
Unit cell:
a: 38.074Å b: 42.539Å c: 72.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.223
Expression system: Escherichia coli BL21(DE3)