4xgq

X-ray diffraction
2.7Å resolution

Crystal structure of addiction module from Mycobacterial species

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease VapC30 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 132 amino acids
Theoretical weight: 14.4 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WF77 (Residues: 1-131; Coverage: 100%)
Gene names: MTCY20H10.05, Rv0624, vapC30
Sequence domains: PIN domain
Structure domains: 5'-nuclease
Antitoxin VapB30 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 84 amino acids
Theoretical weight: 9.16 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJ35 (Residues: 1-84; Coverage: 100%)
Gene names: Rv0623, vapB30
Sequence domains: Rv0623-like transcription factor

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P3121
Unit cell:
a: 96.381Å b: 96.381Å c: 232.788Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.206 0.238
Expression system: Escherichia coli