4xgc

X-ray diffraction
3.5Å resolution

Crystal structure of the eukaryotic origin recognition complex

Released:
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of the eukaryotic origin recognition complex.
OpenAccess logo Nature 519 321-6 (2015)
PMID: 25762138

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.11 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24168 (Residues: 266-618; Coverage: 57%)
Gene names: CG3041, Orc2
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 676 amino acids
Theoretical weight: 77.65 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7K2L1 (Residues: 46-721; Coverage: 94%)
Gene names: CG34315-RB, CG4088, DmORC3, DmOrc3, Dmel\CG4088, Dmel_CG4088, LAT, Lat, ORC, ORC3, Orc3, l(2)49Fd, l(2)vr6, lat, lat-RA, latheo, orc3, vr-6, vr6
Sequence domains:
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 460 amino acids
Theoretical weight: 52.18 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24169 (Residues: 1-460; Coverage: 100%)
Gene names: CG7833, Orc5
Sequence domains:
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 43.91 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O16810 (Residues: 532-924; Coverage: 43%)
Gene names: CG10667, Orc1
Sequence domains:
Origin recognition complex subunit 2 Chain: G
Molecule details ›
Chain: G
Length: 41 amino acids
Theoretical weight: 3.51 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
Origin recognition complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 72 amino acids
Theoretical weight: 8.2 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y1B2 (Residues: 187-257; Coverage: 28%)
Gene names: CG1584, Orc6
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 459 amino acids
Theoretical weight: 52 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W102 (Residues: 1-459; Coverage: 100%)
Gene names: CG2917, DmORC4, Dmel\CG2917, Dmel_CG2917, ORC, ORC4, Orc4, dmOrc4, orc4, rDmORC
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 145.545Å b: 258.983Å c: 257.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.258
Expression system: Trichoplusia ni