4x4u

X-ray diffraction
2.7Å resolution

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a human MenBeta minihelix ending in CCACC

Released:

Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
CCA-adding enzyme Chains: A, C, E, F
Molecule details ›
Chains: A, C, E, F
Length: 457 amino acids
Theoretical weight: 53.67 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:
human MenBeta minihelix Chains: B, D, G
Molecule details ›
Chains: B, D, G
Length: 37 nucleotides
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-D(*GP*G)-3') Chain: H
Molecule details ›
Chain: H
Length: 2 nucleotides
Theoretical weight: 645 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 114.988Å b: 84.049Å c: 135.524Å
α: 90° β: 103.42° γ: 90°
R-values:
R R work R free
0.185 0.183 0.233
Expression systems:
  • Escherichia coli
  • Not provided