4x4p

X-ray diffraction
3Å resolution

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCAC

Released:

Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CCA-adding enzyme Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 457 amino acids
Theoretical weight: 53.67 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:
G70A tRNA minihelix ending in CCAC Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 36 nucleotides
Theoretical weight: 11.56 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 58.108Å b: 58.112Å c: 217.806Å
α: 85.2° β: 87.98° γ: 89.81°
R-values:
R R work R free
0.226 0.224 0.274
Expression systems:
  • Escherichia coli
  • Not provided