4x4e

X-ray diffraction
2.8Å resolution

RADIATION DAMAGE TO THE NUCLEOPROTEIN COMPLEX C.Esp1396I: DOSE (DWD) 14.4 MGy

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 82 amino acids
Theoretical weight: 9.52 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
35-MER DNA Chain: E
Molecule details ›
Chain: E
Length: 35 nucleotides
Theoretical weight: 10.79 KDa
Source organism: synthetic construct
Expression system: Not provided
35-MER DNA Chain: F
Molecule details ›
Chain: F
Length: 35 nucleotides
Theoretical weight: 10.74 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 104.44Å b: 104.44Å c: 139.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.24 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided