› 4wh9

X-ray diffraction
1.5Å resolution

Structure of the CDC25B Phosphatase Catalytic Domain with Bound Inhibitor

Released:
DOI: 10.2210/pdb4wh9/pdb
PDB entry 4wh9 coloured by chain, front view.
PDB entry 4wh9 coloured by chain, side view.
PDB entry 4wh9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition of CDC25B phosphatase through disruption of protein-protein interaction.
Lund G, Dudkin S, Borkin D, Ni W, Grembecka J, Cierpicki T
ACS Chem Biol 10 390-4 (2015)
PMID: 25423142

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30305 (Residues: 386-565; Coverage: 31%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

3 bound ligands:
Ligand 3M8 1 x 3M8
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 51.66Å b: 71.47Å c: 73.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 0.126 0.152
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phosphatases and kinases regulating CDC25 activity in the cell cycle: clinical implications of CDC25 overexpression and potential treatment strategies.
Sur et al. (2016)
4 more

4 mentions without citation

Inhibition of CDC25B phosphatase through disruption of protein-protein interaction.
Lund et al. (2015)
3 more