PDBe 4v49

X-ray diffraction
8.7Å resolution

Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli 70S Ribosome.

Released:
Source organism: Escherichia coli

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
Entry contents:
50 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (55 distinct):
30S ribosomal protein S2 Chain: AB
Molecule details ›
Chain: AB
Length: 234 amino acids
Theoretical weight: 26.99 KDa
Source organism: Escherichia coli
30S ribosomal protein S3 Chain: AC
Molecule details ›
Chain: AC
Length: 206 amino acids
Theoretical weight: 22.86 KDa
Source organism: Escherichia coli
30S ribosomal protein S4 Chain: AD
Molecule details ›
Chain: AD
Length: 208 amino acids
Theoretical weight: 24.24 KDa
Source organism: Escherichia coli
30S ribosomal protein S5 Chain: AE
Molecule details ›
Chain: AE
Length: 150 amino acids
Theoretical weight: 16.33 KDa
Source organism: Escherichia coli
30S ribosomal protein S6 Chain: AF
Molecule details ›
Chain: AF
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Escherichia coli
30S ribosomal protein S7 Chain: AG
Molecule details ›
Chain: AG
Length: 155 amino acids
Theoretical weight: 17.92 KDa
Source organism: Escherichia coli
30S ribosomal protein S8 Chain: AH
Molecule details ›
Chain: AH
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Escherichia coli
30S ribosomal protein S9 Chain: AI
Molecule details ›
Chain: AI
Length: 127 amino acids
Theoretical weight: 14.3 KDa
Source organism: Escherichia coli
50S ribosomal protein L2 Chain: BA
Molecule details ›
Chain: BA
Length: 270 amino acids
Theoretical weight: 29.52 KDa
Source organism: Escherichia coli
50S ribosomal protein L3 Chain: BB
Molecule details ›
Chain: BB
Length: 205 amino acids
Theoretical weight: 21.96 KDa
Source organism: Escherichia coli
50S ribosomal protein L4 Chain: BC
Molecule details ›
Chain: BC
Length: 197 amino acids
Theoretical weight: 21.41 KDa
Source organism: Escherichia coli
50S ribosomal protein L5 Chain: BD
Molecule details ›
Chain: BD
Length: 178 amino acids
Theoretical weight: 20.12 KDa
Source organism: Escherichia coli
50S ribosomal protein L6 Chain: BE
Molecule details ›
Chain: BE
Length: 177 amino acids
Theoretical weight: 18.68 KDa
Source organism: Escherichia coli
50S ribosomal protein L9 Chain: BF
Molecule details ›
Chain: BF
Length: 52 amino acids
Theoretical weight: 5.73 KDa
Source organism: Escherichia coli
30S ribosomal protein S10 Chain: AJ
Molecule details ›
Chain: AJ
Length: 98 amino acids
Theoretical weight: 11.3 KDa
Source organism: Escherichia coli
30S ribosomal protein S11 Chain: AK
Molecule details ›
Chain: AK
Length: 119 amino acids
Theoretical weight: 12.61 KDa
Source organism: Escherichia coli
30S ribosomal protein S12 Chain: AL
Molecule details ›
Chain: AL
Length: 124 amino acids
Theoretical weight: 13.8 KDa
Source organism: Escherichia coli
30S ribosomal protein S13 Chain: AM
Molecule details ›
Chain: AM
Length: 125 amino acids
Theoretical weight: 14.21 KDa
Source organism: Escherichia coli
30S ribosomal protein S14 Chain: AN
Molecule details ›
Chain: AN
Length: 60 amino acids
Theoretical weight: 7.03 KDa
Source organism: Escherichia coli
30S ribosomal protein S15 Chain: AO
Molecule details ›
Chain: AO
Length: 88 amino acids
Theoretical weight: 10.45 KDa
Source organism: Escherichia coli
30S ribosomal protein S16 Chain: AP
Molecule details ›
Chain: AP
Length: 83 amino acids
Theoretical weight: 9.92 KDa
Source organism: Escherichia coli
30S ribosomal protein S17 Chain: AQ
Molecule details ›
Chain: AQ
Length: 104 amino acids
Theoretical weight: 12.19 KDa
Source organism: Escherichia coli
30S ribosomal protein S18 Chain: AR
Molecule details ›
Chain: AR
Length: 73 amino acids
Theoretical weight: 8.48 KDa
Source organism: Escherichia coli
30S ribosomal protein S19 Chain: AS
Molecule details ›
Chain: AS
Length: 80 amino acids
Theoretical weight: 9.2 KDa
Source organism: Escherichia coli
30S ribosomal protein S20 Chain: AT
Molecule details ›
Chain: AT
Length: 99 amino acids
Theoretical weight: 10.91 KDa
Source organism: Escherichia coli
50S RIBOSOMAL PROTEIN L28 Chain: BV
Molecule details ›
Chain: BV
Length: 16 amino acids
Theoretical weight: 1.38 KDa
Source organism: Escherichia coli
50S ribosomal protein L11 Chain: BG
Molecule details ›
Chain: BG
Length: 143 amino acids
Theoretical weight: 14.82 KDa
Source organism: Escherichia coli
50S ribosomal protein L13 Chain: BH
Molecule details ›
Chain: BH
Length: 143 amino acids
Theoretical weight: 15.94 KDa
Source organism: Escherichia coli
50S ribosomal protein L14 Chain: BI
Molecule details ›
Chain: BI
Length: 132 amino acids
Theoretical weight: 14.01 KDa
Source organism: Escherichia coli
50S ribosomal protein L15 Chain: BJ
Molecule details ›
Chain: BJ
Length: 141 amino acids
Theoretical weight: 15.18 KDa
Source organism: Escherichia coli
50S ribosomal protein L16 Chain: BK
Molecule details ›
Chain: BK
Length: 124 amino acids
Theoretical weight: 14.03 KDa
Source organism: Escherichia coli
50S ribosomal protein L17 Chain: BL
Molecule details ›
Chain: BL
Length: 114 amino acids
Theoretical weight: 12.64 KDa
Source organism: Escherichia coli
50S ribosomal protein L18 Chain: BM
Molecule details ›
Chain: BM
Length: 111 amino acids
Theoretical weight: 11.86 KDa
Source organism: Escherichia coli
50S ribosomal protein L19 Chain: BN
Molecule details ›
Chain: BN
Length: 125 amino acids
Theoretical weight: 14.32 KDa
Source organism: Escherichia coli
50S ribosomal protein L1P Chain: B5
Molecule details ›
Chain: B5
Length: 217 amino acids
Theoretical weight: 24.69 KDa
Source organism: Escherichia coli
50S ribosomal protein L20 Chain: BO
Molecule details ›
Chain: BO
Length: 117 amino acids
Theoretical weight: 13.86 KDa
Source organism: Escherichia coli
50S ribosomal protein L21 Chain: BP
Molecule details ›
Chain: BP
Length: 100 amino acids
Theoretical weight: 11.17 KDa
Source organism: Escherichia coli
50S ribosomal protein L22 Chain: BQ
Molecule details ›
Chain: BQ
Length: 130 amino acids
Theoretical weight: 14.79 KDa
Source organism: Escherichia coli
50S ribosomal protein L23 Chain: BR
Molecule details ›
Chain: BR
Length: 93 amino acids
Theoretical weight: 10.34 KDa
Source organism: Escherichia coli
50S ribosomal protein L24 Chain: BS
Molecule details ›
Chain: BS
Length: 113 amino acids
Theoretical weight: 12.14 KDa
Source organism: Escherichia coli
50S ribosomal protein L27 Chain: BU
Molecule details ›
Chain: BU
Length: 86 amino acids
Theoretical weight: 9.12 KDa
Source organism: Escherichia coli
50S ribosomal protein L29 Chain: BW
Molecule details ›
Chain: BW
Length: 65 amino acids
Theoretical weight: 7.52 KDa
Source organism: Escherichia coli
50S ribosomal protein L30 Chain: BX
Molecule details ›
Chain: BX
Length: 55 amino acids
Theoretical weight: 6.08 KDa
Source organism: Escherichia coli
50S ribosomal protein L31 Chain: BY
Molecule details ›
Chain: BY
Length: 73 amino acids
Theoretical weight: 8.6 KDa
Source organism: Escherichia coli
50S ribosomal protein L32 Chain: BZ
Molecule details ›
Chain: BZ
Length: 58 amino acids
Theoretical weight: 6.58 KDa
Source organism: Escherichia coli
50S ribosomal protein L33 Chain: B1
Molecule details ›
Chain: B1
Length: 53 amino acids
Theoretical weight: 6.14 KDa
Source organism: Escherichia coli
50S ribosomal protein L34 Chain: B2
Molecule details ›
Chain: B2
Length: 46 amino acids
Theoretical weight: 5.5 KDa
Source organism: Escherichia coli
50S ribosomal protein L35 Chain: B3
Molecule details ›
Chain: B3
Length: 63 amino acids
Theoretical weight: 7.13 KDa
Source organism: Escherichia coli
50S ribosomal protein L36 Chain: B4
Molecule details ›
Chain: B4
Length: 35 amino acids
Theoretical weight: 4.13 KDa
Source organism: Escherichia coli
general stress protein Ctc Chain: BT
Molecule details ›
Chain: BT
Length: 173 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
tRNA-Phe Chains: AV, AW
Molecule details ›
Chains: AV, AW
Length: 76 nucleotides
Theoretical weight: 24.52 KDa
Messenger RNA Chain: AU
Molecule details ›
Chain: AU
Length: 18 nucleotides
Theoretical weight: 5.73 KDa
5S ribosomal RNA Chain: B9
Molecule details ›
Chain: B9
Length: 118 nucleotides
Theoretical weight: 38.03 KDa
16S ribosomal RNA Chain: AA
Molecule details ›
Chain: AA
Length: 1527 nucleotides
Theoretical weight: 495.74 KDa
23S ribosomal RNA Chain: B0
Molecule details ›
Chain: B0
Length: 2887 nucleotides
Theoretical weight: 935.78 KDa

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I422
Unit cell:
a: 682.32Å b: 682.32Å c: 386.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.394 0.394 0.415